Microbiological and Biochemical Contamination Analysis of Refilled Drinking-water in Abeli, Kendari, Southeast Sulawesi

Microbiological and Biochemical Contamination Analysis of Refilled Drinking-water in Abeli, Kendari, Southeast Sulawesi

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BACKGROUND: The access to safe drinkingwater is increasingly difficult, especially in water catchment or coastal areas.Due to the difficulty, people in coastal areas tend to consume ready-to-use refilled drinking-water.However, the sanitation facilities on most drinking-water refill depots do not meet the requirements, hence really prone to microbial contamination.

We conducted this study to determine the types of bacteria causing the contamination of refilled drinking-water from refill depots in Abeli, a coastal area in Kendari.METHODS: Samples were collected from all 6 drinkingwater refill depots in Abeli.Three-series fermentation tubes were color block iphone case used for the microbiological test, including the presumptive, confirmative and completed tests.

The biochemical test was performed with indole test, methyl red test, Voges-Proskauer test, and citrate utilization test (IMViC) methods and the triple sugar iron agar (TSIA) test.RESULTS: Among 6 refilled drinking-water samples, we cashel tail bag found the growth of coliform bacterial colonies in 3 samples, exhibiting various characteristics.Based on the characteristics, both microbiological and biochemical, the identified bacteria were Proteus sp.

, Escherichia coli and Klebsiella oxytoca.CONCLUSION: Bacteria that contaminated refilled drinking-water in Abeli were Proteus sp., E.

coli and K.oxytoca.Continuous supervision is required to ensure the quality of water from drinking-water refill depots.

KEYWORDS: bacteria, Proteus sp., Escherichia coli, Klebsiella oxytoca, coastal areas, refilled drinking water.